| Gene | Hnrnph2 |
| Chromosome | X |
| Deletion Start | 134,604,893 |
| Deletion End | 134,606,265 |
| Deletion Size | 1,373 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 134,605,024 | 134,605,045 | CACATCAGGTGTTCGTTTCATC |
| TUP | 134,605,051 | 134,605,075 | CAGAGAAGGCAGACCGAGTGGTGAA |
| TUR | 134,605,084 | 134,605,108 | CACTTCATCTTCAGATTCAAGTTCG |
| TDF | 134,605,830 | 134,605,854 | TTTTCTCACCTCTTAATCCCATGAG |
| TDP | 134,605,873 | 134,605,900 | ACCTGATGGAAGAGTTACTGGTGAGGCA |
| TDR | 134,605,979 | 134,606,001 | GCGGTAGAATTCAAGAAGAGCTC |
PCR Primers
| Primer | Sequence |
| SU | AGCACTCGCTAAGTGAATGG |
| SD | TGACAGTTATGGTCAATACTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 333 bp | - | + | + |
| TUF | TUR | 85 bp | + | + | - |
| TDF | TDR | 172 bp | + | + | - |
| NeoFwd | SD | 415 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11671B-C7 | VGB6 | C57BL/6NTac | |
| 11671B-F11 | VGB6 | C57BL/6NTac | |
| 11671C-A9 | VGB6 | C57BL/6NTac | |
| 11671C-H4 | VGB6 | C57BL/6NTac | |
