| Gene | Cdc20 |
| Chromosome | 4 |
| Deletion Start | 118,437,118 |
| Deletion End | 118,432,943 |
| Deletion Size | 4,176 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 118,436,550 | 118,436,570 | TTTGATGTGGAGGAAGCCAAG |
| TUP | 118,436,526 | 118,436,548 | TCCTCAGGCTCAGCGGCAAACCT |
| TUR | 118,436,483 | 118,436,502 | GCCTCGATGATGTCACTCAC |
| TDF | 118,433,467 | 118,433,488 | GCTCAAAGGTAGGTGGCAATAG |
| TDP | 118,433,440 | 118,433,465 | AAGCCAGGCAGAAAGGCCACATAGTG |
| TDR | 118,433,383 | 118,433,403 | CCCAGGCAAGTCATATCAGTC |
PCR Primers
| Primer | Sequence |
| SU | TTAAGAACAACAGTCCGGCG |
| SD | AGTGCATGCGGTAATTCCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 321 bp | - | + | + |
| TUF | TUR | 88 bp | + | + | - |
| TDF | TDR | 106 bp | + | + | - |
| NeoFwd | SD | 348 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11674A-A10 | VGB6 | C57BL/6NTac | |
| 11674A-D2 | VGB6 | C57BL/6NTac | |
| 11674A-C9 | VGB6 | C57BL/6NTac | |
