| Gene | Mrpl35 |
| Chromosome | 6 |
| Deletion Start | 71,823,755 |
| Deletion End | 71,816,025 |
| Deletion Size | 7,731 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 71,823,581 | 71,823,598 | GCGCGATACCTTGCACAG |
| TUP | 71,823,560 | 71,823,580 | TGTAGTGCCCATGACCCGCGT |
| TUR | 71,823,504 | 71,823,525 | TCACCTCCTCTACCTCAGTCTC |
| TDF | 71,816,579 | 71,816,599 | AGAGTGGTGCGTGCTTTCATC |
| TDP | 71,816,553 | 71,816,578 | TGCAGAGCCTGGCTCTCAACTTTCAG |
| TDR | 71,816,528 | 71,816,550 | CACTCAGGAGATTGAGGCAAGAG |
PCR Primers
| Primer | Sequence |
| SU | TTAAGAAGACGGTGGTTTAGG |
| SD | TGGATACCATCTCAATTCCAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 266 bp | - | + | + |
| TUF | TUR | 95 bp | + | + | - |
| TDF | TDR | 72 bp | + | + | - |
| NeoFwd | SD | 318 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11675A-D9 | VGB6 | C57BL/6NTac | |
| 11675A-E9 | VGB6 | C57BL/6NTac | |
| 11675A-E10 | VGB6 | C57BL/6NTac | |
