| Gene | Psmb10 |
| Chromosome | 8 |
| Deletion Start | 105,938,007 |
| Deletion End | 105,935,771 |
| Deletion Size | 2,237 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 105,937,425 | 105,937,445 | TCGCTCACTCCCTACTTTGTC |
| TUP | 105,937,401 | 105,937,423 | CTGCACGTCCCTGTCCACATCCA |
| TUR | 105,937,352 | 105,937,370 | GTGTCCGCAGCTACTCCAG |
| TDF | 105,935,886 | 105,935,906 | GAGCTGGCCGTTACCGATTTG |
| TDP | 105,935,860 | 105,935,883 | CCTGGAACCACACCTGTCCTGACC |
| TDR | 105,935,807 | 105,935,829 | ATGGCCTGCACAGTTTCCTCAAG |
PCR Primers
| Primer | Sequence |
| SU | TGGAGGATCTAAGGCGCAC |
| SD | CTTCTGCACGGATAGATCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 374 bp | - | + | + |
| TUF | TUR | 94 bp | + | + | - |
| TDF | TDR | 100 bp | + | + | - |
| NeoFwd | SD | 284 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11677A-D4 | VGB6 | C57BL/6NTac | |
| 11677A-D9 | VGB6 | C57BL/6NTac | |
| 11677A-E4 | VGB6 | C57BL/6NTac | |
| 11677A-H3 | VGB6 | C57BL/6NTac | |
| 11677A-H10 | VGB6 | C57BL/6NTac | |
| 11677A-H11 | VGB6 | C57BL/6NTac | |
