11679   Cd79b     Status: ES cell colonies screened / QC positives

GeneCd79b
Chromosome11
Deletion Start106,314,481
Deletion End106,311,705
Deletion Size2,777
Genome Build38
MGI IDMGI:96431
NCBI Gene ID15985
BAC ID290K6
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 11

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF106,314,272106,314,295GCTTCTTGTCTGTCTCCCTCTAAC
TUP106,314,249106,314,271TGTCCTGTGCCCAGTTTGTGGGT
TUR106,314,212106,314,230GGGCTGGGACACCTTTGAC
TDF106,311,825106,311,848GAGGTAAAGTGGTCGGTAGGAGAG
TDP106,311,799106,311,823ATCCAGGCCAGGAATGAGGGTCACC
TDR106,311,765106,311,784GAAGCAGTGAGGCCCAAGAG

PCR Primers

PrimerSequence
SUCTCTTCCAGAGCAAGGCAAC
SDTGATCCTTGGAATGGGAGAG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR84 bp++-
TDFTDR84 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
11679A-C2VGB6C57BL/6NTac
11679A-C4VGB6C57BL/6NTac
11679A-C6VGB6C57BL/6NTac
11679A-G6VGB6C57BL/6NTac
11679A-D9VGB6C57BL/6NTac
11679A-G3VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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