| Gene | Lyrm1 |
| Chromosome | 7 |
| Deletion Start | 119,909,788 |
| Deletion End | 119,916,279 |
| Deletion Size | 6,492 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 119,910,038 | 119,910,059 | AACTGAAGAAGACCCATCCAAC |
| TUP | 119,910,060 | 119,910,081 | TCCCAGAACCAGGAAGGTCCCA |
| TUR | 119,910,082 | 119,910,100 | GCCTAGCCAAGCTGCATTC |
| TDF | 119,915,489 | 119,915,512 | CCATACTGTAAGCGAGCCATACTG |
| TDP | 119,915,522 | 119,915,551 | TCTACCTCAGTAAACATTTGAGTTGGCCCA |
| TDR | 119,915,564 | 119,915,582 | GTGCTTGGGTGCGAAGATG |
PCR Primers
| Primer | Sequence |
| SU | TCACTGATGTTTCTACTCTGC |
| SD | TTCAGCCTTCCTCTGTAGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 276 bp | - | + | + |
| TUF | TUR | 63 bp | + | + | - |
| TDF | TDR | 94 bp | + | + | - |
| NeoFwd | SD | 331 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11680A-A4 | VGB6 | C57BL/6NTac | |
| 11680A-B1 | VGB6 | C57BL/6NTac | |
| 11680A-D3 | VGB6 | C57BL/6NTac | |
