| Gene | Tnfaip8l2 |
| Chromosome | 3 |
| Deletion Start | 95,140,547 |
| Deletion End | 95,139,968 |
| Deletion Size | 580 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 95,140,335 | 95,140,356 | GGTAGCGGTTAAAGTGGCTGTG |
| TUP | 95,140,314 | 95,140,333 | TGCACCGCAGTGGCTGCTTT |
| TUR | 95,140,267 | 95,140,291 | CGTAGCTTCTGACGAAATCGTGTAG |
| TDF | 95,140,137 | 95,140,156 | AGCACCACCTCACACCCAAG |
| TDP | 95,140,113 | 95,140,135 | CACATGACCGCATCAGGCACGTG |
| TDR | 95,140,055 | 95,140,076 | GAGTGAAGTCAGGCCCATAGAG |
PCR Primers
| Primer | Sequence |
| SU | TCCTCCAGCCAGGCTCTC |
| SD | GACCTGAGTTTCACTTCTTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 350 bp | - | + | + |
| TUF | TUR | 90 bp | + | + | - |
| TDF | TDR | 102 bp | + | + | - |
| NeoFwd | SD | 329 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11681A-A2 | VGB6 | C57BL/6NTac | |
| 11681A-C5 | VGB6 | C57BL/6NTac | |
| 11681B-B4 | VGB6 | C57BL/6NTac | |
| 11681B-C5 | VGB6 | C57BL/6NTac | |
| 11681B-C6 | VGB6 | C57BL/6NTac | |
| 11681B-A2 | VGB6 | C57BL/6NTac | |
| 11681B-F10 | VGB6 | C57BL/6NTac | |
| 11681B-H10 | VGB6 | C57BL/6NTac | |
