| Gene | Iscu |
| Chromosome | 5 |
| Deletion Start | 113,772,814 |
| Deletion End | 113,777,925 |
| Deletion Size | 5,112 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 113,773,272 | 113,773,293 | TGACAGGAGTCTTCTGAAGTCC |
| TUP | 113,773,306 | 113,773,329 | CGCTCGGTCCAACCAAGGGAAACC |
| TUR | 113,773,375 | 113,773,393 | GGCTAGGTGCTTGGCTGAG |
| TDF | 113,777,806 | 113,777,829 | AGCTGTCTACCTTCCCATGCCTTC |
| TDP | 113,777,833 | 113,777,854 | TGCTGGCAGAAGACGCCATCAA |
| TDR | 113,777,883 | 113,777,905 | GGCTCCTCCTTCTTGGACTCTTG |
PCR Primers
| Primer | Sequence |
| SU | TCGAATCATAAACACGCCTG |
| SD | CTGGCAATCTACCTCAGGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 353 bp | - | + | + |
| TUF | TUR | 122 bp | + | + | - |
| TDF | TDR | 100 bp | + | + | - |
| NeoFwd | SD | 338 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11691A-A12 | VGB6 | C57BL/6NTac | |
| 11691A-B10 | VGB6 | C57BL/6NTac | |
| 11691A-B11 | VGB6 | C57BL/6NTac | |
| 11691A-H8 | VGB6 | C57BL/6NTac | |
