| Gene | Irf9 |
| Chromosome | 14 |
| Deletion Start | 55,605,038 |
| Deletion End | 55,609,036 |
| Deletion Size | 3,999 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 55,605,761 | 55,605,779 | TAGGACACCCCGCTGTCTG |
| TUP | 55,605,781 | 55,605,802 | AAGACTCGCCTACGCTGTGCCC |
| TUR | 55,605,810 | 55,605,832 | GAACCTCCTCAAATTCGGAACTC |
| TDF | 55,608,517 | 55,608,535 | CATCTGGCCTGGACCTCAC |
| TDP | 55,608,545 | 55,608,572 | CAGGCTACTTCAAACATGGCAACTCCGC |
| TDR | 55,608,584 | 55,608,603 | CGCCCATAACCCTAGCACTC |
PCR Primers
| Primer | Sequence |
| SU | GAGAGAAAAGAACATGGAAGG |
| SD | GTATCCTCAACAAGTCTGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 381 bp | - | + | + |
| TUF | TUR | 72 bp | + | + | - |
| TDF | TDR | 87 bp | + | + | - |
| NeoFwd | SD | 240 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11692A-H10 | VGB6 | C57BL/6NTac | |
| 11692A-A3 | VGB6 | C57BL/6NTac | |
| 11692A-B6 | VGB6 | C57BL/6NTac | |
| 11692A-F9 | VGB6 | C57BL/6NTac | |
| 11692A-H2 | VGB6 | C57BL/6NTac | |
