| Gene | Ifnar2 |
| Chromosome | 16 |
| Deletion Start | 91,383,902 |
| Deletion End | 91,404,482 |
| Deletion Size | 20,601 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 91,384,729 | 91,384,747 | CCCTGCCGTTTGTGTCAAG |
| TUP | 91,384,748 | 91,384,771 | TCCCAGCGGCCTCACTTTACATCC |
| TUR | 91,384,783 | 91,384,801 | CGTGTGCCATCTGAAGGTG |
| TDF | 91,403,889 | 91,403,907 | ACTGACGGGCAAGCCTCTG |
| TDP | 91,403,911 | 91,403,935 | CAAACCTCTGACACCTCAGCCAGCC |
| TDR | 91,403,956 | 91,403,974 | CCTCAGCGCCTGAATCTTC |
PCR Primers
| Primer | Sequence |
| SU | CAGGGATCAAGGAAGGACTG |
| SD | GCCTTACCTCTGACCCTTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 521 bp | - | + | + |
| TUF | TUR | 73 bp | + | + | - |
| TDF | TDR | 86 bp | + | + | - |
| NeoFwd | SD | 440 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11700A-A4 | VGB6 | C57BL/6NTac | |
| 11700A-F10 | VGB6 | C57BL/6NTac | |
