| Gene | Tmem125 |
| Chromosome | 4 |
| Deletion Start | 118,543,131 |
| Deletion End | 118,542,441 |
| Deletion Size | 710 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 118,541,990 | 118,542,009 | CCCTGCTGGTTCTGGTGAAG |
| TUP | 118,541,966 | 118,541,989 | CAGCTGATGAGCTCTGCCGTGCAG |
| TUR | 118,541,942 | 118,541,960 | GGCCTGGCGTATGCAGTTC |
| TDF | 118,541,780 | 118,541,803 | ATGCTGTCTGTGGGCATTACCTTG |
| TDP | 118,541,755 | 118,541,777 | TTCCTTGGGCTCAGTCTTGCTGC |
| TDR | 118,541,725 | 118,541,746 | TCACACCCACCTGGTACAGTAG |
PCR Primers
| Primer | Sequence |
| SU | TGGCAAGGTGCAGTGCTAG |
| SD | GATCACTAGTGTCGTTGTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 766 bp | - | + | + |
| TUF | TUR | 68 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | 1329 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11701B-B8 | VGB6 | C57BL/6NTac | |
| 11701C-A5 | VGB6 | C57BL/6NTac | |
| 11701C-D9 | VGB6 | C57BL/6NTac | |
| 11701B-H6 | VGB6 | C57BL/6NTac | |
