| Gene | Nr1d1 |
| Chromosome | 11 |
| Deletion Start | 98,774,745 |
| Deletion End | 98,768,169 |
| Deletion Size | 6,577 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 98,774,450 | 98,774,472 | CGAGTGGAAGAGTTTAGGGAGAC |
| TUP | 98,774,417 | 98,774,442 | TTTGAAGCAGTCTCTGGACACGTCCC |
| TUR | 98,774,398 | 98,774,416 | TGCAAGGAGTGGGCAAATG |
| TDF | 98,768,836 | 98,768,859 | GAAGCATGAACCAAGTAAGCACAG |
| TDP | 98,768,810 | 98,768,835 | ACGACTATTTCTGCCCGGGAGAGCTG |
| TDR | 98,768,756 | 98,768,779 | CCTGGCAGTTATCTTGTCCCTTTG |
PCR Primers
| Primer | Sequence |
| SU | GAACTGCTGCAGGCTGATTC |
| SD | GCTCAGCTGTGAACTATTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 380 bp | - | + | + |
| TUF | TUR | 75 bp | + | + | - |
| TDF | TDR | 104 bp | + | + | - |
| NeoFwd | SD | 340 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11705A-E7 | VGB6 | C57BL/6NTac | |
