| Gene | Aarsd1 |
| Chromosome | 11 |
| Deletion Start | 101,424,620 |
| Deletion End | 101,419,112 |
| Deletion Size | 5,509 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 101,424,538 | 101,424,560 | GCAGATTTCCTCTCCCACATTTG |
| TUP | 101,424,516 | 101,424,537 | TAGAAGCCGCTGAGTCTGCCCA |
| TUR | 101,424,483 | 101,424,504 | ACAGTCACAGAGACGTGGAGTC |
| TDF | 101,419,865 | 101,419,883 | TGGAAACCAAAGGCCAGTC |
| TDP | 101,419,827 | 101,419,855 | AGCAGCAAGTGGTCTTAACTGTTGAACCA |
| TDR | 101,419,795 | 101,419,813 | GAGATGGTCCCAGCCAGAG |
PCR Primers
| Primer | Sequence |
| SU | CGAGAACAAGTAACTTCCTGC |
| SD | CATGTCCCAACACCCAATCA |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 405 bp | - | + | + |
| TUF | TUR | 78 bp | + | + | - |
| TDF | TDR | 89 bp | + | + | - |
| NeoFwd | SD | 338 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11711A-C6 | VGB6 | C57BL/6NTac | |
| 11711A-E9 | VGB6 | C57BL/6NTac | |
