| Gene | Aqp3 |
| Chromosome | 4 |
| Deletion Start | 41,097,403 |
| Deletion End | 41,092,801 |
| Deletion Size | 4,603 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 41,097,689 | 41,097,710 | GTTTCCTGGCTTCCCAACTTTC |
| TUP | 41,097,667 | 41,097,688 | CCAGGGCTCCAACCTCAGGACA |
| TUR | 41,097,641 | 41,097,661 | GGGTGTCCATTTGCCTTTCTC |
| TDF | 41,094,296 | 41,094,315 | CCACCTATCCCTCTGGACAC |
| TDP | 41,094,267 | 41,094,294 | TGGACATGGTCAATGGCTTCTTTGATCA |
| TDR | 41,094,224 | 41,094,248 | GTTCCTCCCTCTACCTTTACTTCAC |
PCR Primers
| Primer | Sequence |
| SU | TCACTCCCCTAAGAATCCTG |
| SD | GAAAACAGCACACATGTACAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 70 bp | + | + | - |
| TDF | TDR | 92 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11716A-A1 | VGB6 | C57BL/6NTac | |
| 11716A-A5 | VGB6 | C57BL/6NTac | |
| 11716A-C4 | VGB6 | C57BL/6NTac | |
| 11716A-D6 | VGB6 | C57BL/6NTac | |
| 11716A-C9 | VGB6 | C57BL/6NTac | |
