| Gene | Fam43a |
| Chromosome | 16 |
| Deletion Start | 30,600,603 |
| Deletion End | 30,601,876 |
| Deletion Size | 1,274 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 30,600,751 | 30,600,770 | GCAGCATGTTCCGCTCCAAG |
| TUP | 30,600,771 | 30,600,794 | CGCAAGAAGCTGCACATCACCAGC |
| TUR | 30,600,805 | 30,600,826 | CCCAGGTAGAGCACAGTGTAAG |
| TDF | 30,601,612 | 30,601,634 | GGACATCACTTCCTCTGTCAGTC |
| TDP | 30,601,637 | 30,601,659 | TGCCTCAGACATGAAGGCCCAAC |
| TDR | 30,601,700 | 30,601,719 | CCAGGGTGCTCACGTCATTG |
PCR Primers
| Primer | Sequence |
| SU | ATCCTACCCCAGTCCCTGC |
| SD | AACCTGAGCCACCTCTGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 76 bp | + | + | - |
| TDF | TDR | 108 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11717A-B2 | VGB6 | C57BL/6NTac | |
| 11717A-B11 | VGB6 | C57BL/6NTac | |
| 11717A-G3 | VGB6 | C57BL/6NTac | |
| 11717A-F3 | VGB6 | C57BL/6NTac | |
| 11717A-E11 | VGB6 | C57BL/6NTac | |
| 11717A-C6 | VGB6 | C57BL/6NTac | |
