| Gene | Dio1 |
| Chromosome | 4 |
| Deletion Start | 107,307,114 |
| Deletion End | 107,292,293 |
| Deletion Size | 14,822 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 107,307,043 | 107,307,061 | GCCTTGGAGGTGGCTGTAG |
| TUP | 107,307,015 | 107,307,041 | CAAGGTGCTAATGACGCTGTTCCCAGG |
| TUR | 107,306,991 | 107,307,010 | GCCAGGATGCTCTGTTTGAC |
| TDF | 107,292,399 | 107,292,419 | AAAGCTGGCCCTTGGAACTAC |
| TDP | 107,292,372 | 107,292,396 | TCCTGAGGAAGTCCGAGCTGTCCTG |
| TDR | 107,292,344 | 107,292,365 | CGTGTCTAGGTGGAGTGCAAAG |
PCR Primers
| Primer | Sequence |
| SU | TAACAAAGAATCCGGGAGGG |
| SD | CATCCAAGCATGTGAGTGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 71 bp | + | + | - |
| TDF | TDR | 76 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11718D-F7 | VGB6 | C57BL/6NTac | |
| 11718D-G6 | VGB6 | C57BL/6NTac | |
| 11718E-A4 | VGB6 | C57BL/6NTac | |
| 11718E-B11 | VGB6 | C57BL/6NTac | |
| 11718E-C2 | VGB6 | C57BL/6NTac | |
