| Gene | Sub1 |
| Chromosome | 15 |
| Deletion Start | 11,994,122 |
| Deletion End | 11,984,481 |
| Deletion Size | 9,642 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 11,993,132 | 11,993,152 | TCCAGTTGATCTAGCCTGCTG |
| TUP | 11,993,109 | 11,993,131 | TGGCGCCTCGTGTGAGTTTGATC |
| TUR | 11,993,077 | 11,993,098 | GGGTCTCTTCTCCTACCATGTG |
| TDF | 11,984,869 | 11,984,892 | GCTGAGTGTCACTCTTGATAGCAC |
| TDP | 11,984,840 | 11,984,864 | CCAGAGTGCACCCATACCCATCTGA |
| TDR | 11,984,766 | 11,984,788 | TCTTCCATCAGCATCCATTTCAC |
PCR Primers
| Primer | Sequence |
| SU | GCTTTCTTGTAGTCTACCCAC |
| SD | CAACAGTGTGCCTAGTTGTTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 76 bp | + | + | - |
| TDF | TDR | 127 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11720A-D12 | VGB6 | C57BL/6NTac | |
| 11720A-E3 | VGB6 | C57BL/6NTac | |
