| Gene | Gc |
| Chromosome | 5 |
| Deletion Start | 89,446,564 |
| Deletion End | 89,422,045 |
| Deletion Size | 24,520 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 89,446,388 | 89,446,408 | TGGGCTCTGAGATGAGGGAAG |
| TUP | 89,446,359 | 89,446,386 | CCATTGAGTTGCTGTGGACCAACAGGTC |
| TUR | 89,446,339 | 89,446,356 | GCTGGCACCTCTGCTTAC |
| TDF | 89,422,864 | 89,422,882 | AAAGCCCACACCTGCCAAC |
| TDP | 89,422,832 | 89,422,861 | TGCCTTCCAAATATCTGTCAGTACCCATCC |
| TDR | 89,422,784 | 89,422,805 | CCAAGGACAGACTACAGCAAAC |
PCR Primers
| Primer | Sequence |
| SU | GTTCTGACTCACTGACGTGG |
| SD | GAAGCACACAGCTCTGGAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 70 bp | + | + | - |
| TDF | TDR | 99 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11723A-B3 | VGB6 | C57BL/6NTac | |
| 11723A-F2 | VGB6 | C57BL/6NTac | |
