| Gene | Elovl1 |
| Chromosome | 4 |
| Deletion Start | 118,430,513 |
| Deletion End | 118,432,143 |
| Deletion Size | 1,631 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 118,430,550 | 118,430,572 | ATGCGGGTAAGCTGCTGTCTATG |
| TUP | 118,430,576 | 118,430,600 | CTGCCTTCCTTCCCTTCTTCCCTGG |
| TUR | 118,430,625 | 118,430,648 | GTCAGGTCACAAGGAGTTCCTAAG |
| TDF | 118,431,571 | 118,431,592 | CCTGACCATCTTGTTTGCCATC |
| TDP | 118,431,595 | 118,431,618 | AGGAGGAATGGGCTCCTTCCATGC |
| TDR | 118,431,663 | 118,431,682 | AGGGCCAAGGGCAGACAATC |
PCR Primers
| Primer | Sequence |
| SU | CACTCGAAGAGAGTGGAGGG |
| SD | GTGAGGAGAAAGGCCACAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 99 bp | + | + | - |
| TDF | TDR | 112 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11725A-F9 | VGB6 | C57BL/6NTac | |
| 11725A-G6 | VGB6 | C57BL/6NTac | |
| 11725A-H6 | VGB6 | C57BL/6NTac | |
