11729   Mrpl51     Status: ES cell colonies screened / QC positives

GeneMrpl51
Chromosome6
Deletion Start125,192,358
Deletion End125,193,424
Deletion Size1,067
Genome Build38
MGI IDMGI:1913743
NCBI Gene ID66493
BAC ID218K6
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 6

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF125,192,490125,192,511TGATTCATGCTTCTGTCCTTCC
TUP125,192,512125,192,534CTAGGCGTCCCACGGTTGGCCTT
TUR125,192,574125,192,593GAGCCCTCTTCTCGTTCCAG
TDF125,192,959125,192,980ACCGTGTACTGCTCAAGCAATG
TDP125,192,983125,193,007TGCAGGTTCAGGGTGACTTCCTAGA
TDR125,193,014125,193,035AAGGTTCACGACTGCCTGAAAG

PCR Primers

PrimerSequence
SUCAGCGGTTCCATTCTTCCAG
SDCTTACACGAGGTGCCAGTG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR104 bp++-
TDFTDR77 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
11729A-A7VGB6C57BL/6NTac
11729A-C10VGB6C57BL/6NTac
11729A-E5VGB6C57BL/6NTac
11729A-C12VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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