| Gene | Gja4 |
| Chromosome | 4 |
| Deletion Start | 127,313,506 |
| Deletion End | 127,312,499 |
| Deletion Size | 1,008 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 127,312,527 | 127,312,546 | GGACGGTCGTCTTCGGATTC |
| TUP | 127,312,495 | 127,312,520 | CGCTCATGGGTACCTATGTGGTCAGC |
| TUR | 127,312,468 | 127,312,487 | TGCCTCCAGCACACTCTTAC |
| TDF | 127,312,231 | 127,312,250 | TAAAGGCACGAAGGGACCAC |
| TDP | 127,312,194 | 127,312,216 | CCAGGGCAGTGCCTCAGACCCTT |
| TDR | 127,312,110 | 127,312,129 | TGGATGAGAGCCCGTTGTAG |
PCR Primers
| Primer | Sequence |
| SU | TTCTGTTTCCATAGGAACCTC |
| SD | AACACTTAGTCAAGAGCACTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 141 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11730A-B5 | VGB6 | C57BL/6NTac | |
| 11730A-C7 | VGB6 | C57BL/6NTac | |
| 11730A-C9 | VGB6 | C57BL/6NTac | |
| 11730A-C10 | VGB6 | C57BL/6NTac | |
| 11730A-H9 | VGB6 | C57BL/6NTac | |
