| Gene | Rcor2 |
| Chromosome | 19 |
| Deletion Start | 7,269,773 |
| Deletion End | 7,274,657 |
| Deletion Size | 4,885 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 7,270,661 | 7,270,679 | CCGTGGCAGAACCTGAGTG |
| TUP | 7,270,682 | 7,270,704 | TTCTGTCCTGGACCAGGCACTCG |
| TUR | 7,270,736 | 7,270,756 | GCTAGGTCAGCCAGTGATTTC |
| TDF | 7,274,056 | 7,274,077 | CAGAGGTGATTGGGAACAAGAC |
| TDP | 7,274,078 | 7,274,106 | TCTGACCCAAGTGAAGACCTTCTTTGTGA |
| TDR | 7,274,108 | 7,274,127 | GGTTGAAGCGGCGACGATAG |
PCR Primers
| Primer | Sequence |
| SU | CAAAGCACTTTGCTCTAGCC |
| SD | TCCAGTCACAGCCAGGAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 96 bp | + | + | - |
| TDF | TDR | 72 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11734A-A1 | VGB6 | C57BL/6NTac | |
| 11734A-F2 | VGB6 | C57BL/6NTac | |
| 11734A-E1 | VGB6 | C57BL/6NTac | |
