| Gene | Bola1 |
| Chromosome | 3 |
| Deletion Start | 96,197,272 |
| Deletion End | 96,196,788 |
| Deletion Size | 485 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 96,197,168 | 96,197,187 | ACCAGTGGAAGCCGCTATTC |
| TUP | 96,197,144 | 96,197,166 | CGCCAAATTGGAGCAAGCCCTGA |
| TUR | 96,197,066 | 96,197,084 | CCACGCGAAAATGCGTCTC |
| TDF | 96,196,989 | 96,197,008 | ACGAGGCACTGTCGGAGGAG |
| TDP | 96,196,963 | 96,196,984 | CTGGACCGGTACATGCCCTGGC |
| TDR | 96,196,913 | 96,196,933 | TGTCCAACTGTGGGTTTTCTC |
PCR Primers
| Primer | Sequence |
| SU | CAGCCAAAAGGACCAAGTTG |
| SD | CACTAATGATGTGTAGCTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 122 bp | + | + | - |
| TDF | TDR | 96 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11736A-D5 | VGB6 | C57BL/6NTac | |
| 11736A-D7 | VGB6 | C57BL/6NTac | |
