| Gene | Mzt2 |
| Chromosome | 16 |
| Deletion Start | 15,863,197 |
| Deletion End | 15,848,843 |
| Deletion Size | 14,355 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 15,862,259 | 15,862,278 | TGCAGACCCATCCACCTTTC |
| TUP | 15,862,224 | 15,862,251 | TTAGTTGGCTCCACCTTTCGGATTGTAC |
| TUR | 15,862,195 | 15,862,215 | GCTTGCTTGGACCAGTCTTTG |
| TDF | 15,848,983 | 15,849,001 | GGGCTGCCAACAGGTGTAG |
| TDP | 15,848,956 | 15,848,980 | CAGAGTTAGTGCTGGTGCTCCCACA |
| TDR | 15,848,908 | 15,848,929 | AAGATGCTGGCACATTGGTAAG |
PCR Primers
| Primer | Sequence |
| SU | CAGTCCAAGTCTGATCGTCAG |
| SD | TCCCAATTAGTTTGGCTGTAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 84 bp | + | + | - |
| TDF | TDR | 94 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11739A-G5 | VGB6 | C57BL/6NTac | |
| 11739A-D9 | VGB6 | C57BL/6NTac | |
| 11739A-A7 | VGB6 | C57BL/6NTac | |
