| Gene | Pygo2 |
| Chromosome | 3 |
| Deletion Start | 89,431,138 |
| Deletion End | 89,433,514 |
| Deletion Size | 2,377 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 89,431,465 | 89,431,487 | CCAAAGTGATGGGATTCCAGATG |
| TUP | 89,431,488 | 89,431,513 | TGCACCGCCATACTGGTTTCTGTAGT |
| TUR | 89,431,553 | 89,431,574 | AGATGCCTCTCCTCCAGAGTTC |
| TDF | 89,432,763 | 89,432,783 | CCCCTGGCAACATGAACTTTC |
| TDP | 89,432,788 | 89,432,812 | TCAACCCTTCAACCAGTCTCTGGGC |
| TDR | 89,432,842 | 89,432,859 | CCTACTGGGCCTGGCATC |
PCR Primers
| Primer | Sequence |
| SU | TGGATGACACTGAATTGAGAC |
| SD | GGTTAGACTCTAGGAATGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 110 bp | + | + | - |
| TDF | TDR | 97 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11743A-D10 | VGB6 | C57BL/6NTac | |
| 11743A-E10 | VGB6 | C57BL/6NTac | |
| 11743A-F1 | VGB6 | C57BL/6NTac | |
| 11743A-D7 | VGB6 | C57BL/6NTac | |
| 11743A-D4 | VGB6 | C57BL/6NTac | |
