| Gene | Hcrt |
| Chromosome | 11 |
| Deletion Start | 100,762,313 |
| Deletion End | 100,761,263 |
| Deletion Size | 1,051 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 100,762,604 | 100,762,624 | TTCCGATGTTCTCTGTGCTTG |
| TUP | 100,762,569 | 100,762,596 | TGTCTTGCACTCAACAGGGTAGTAGGGA |
| TUR | 100,762,534 | 100,762,554 | AGCCCTTCCTCACCTACAAAC |
| TDF | 100,762,034 | 100,762,053 | CCTGCCGTCTCTACGAACTG |
| TDP | 100,762,013 | 100,762,033 | TTGCACGGAGCTGGCAACCAC |
| TDR | 100,761,984 | 100,762,005 | CGCTTTCCCAGAGTCAGGATAC |
PCR Primers
| Primer | Sequence |
| SU | ATTCTTTGGGTCCAACCTGG |
| SD | ACTGCAAGGTTCCTTGCTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 91 bp | + | + | - |
| TDF | TDR | 70 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11744A-C9 | VGB6 | C57BL/6NTac | |
| 11744A-D11 | VGB6 | C57BL/6NTac | |
| 11744A-H1 | VGB6 | C57BL/6NTac | |
