| Gene | Ubxn10 |
| Chromosome | 4 |
| Deletion Start | 138,735,205 |
| Deletion End | 138,734,343 |
| Deletion Size | 863 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 138,721,156 | 138,721,177 | GCACTATCCTCTTCACCTCCAC |
| TUP | 138,721,130 | 138,721,150 | TCCTCCGGGTCTCCCAGCAAC |
| TUR | 138,721,103 | 138,721,120 | GACCGTTGCACAGACTCC |
| TDF | 138,720,609 | 138,720,628 | AGACGGTTCTCCGACCTCAC |
| TDP | 138,720,584 | 138,720,608 | CAAGTCCCTCCAGGAATGCGGCATC |
| TDR | 138,720,558 | 138,720,580 | GAGATGCCCAACACGGATTTATG |
PCR Primers
| Primer | Sequence |
| SU | AAGTTCCAAGGACTTTGGATC |
| SD | GATGTAGAACTTCCTCATGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 75 bp | + | + | - |
| TDF | TDR | 71 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11750A-C4 | VGB6 | C57BL/6NTac | |
| 11750A-H2 | VGB6 | C57BL/6NTac | |
| 11750A-G4 | VGB6 | C57BL/6NTac | |
| 11750A-F4 | VGB6 | C57BL/6NTac | |
| 11750A-F9 | VGB6 | C57BL/6NTac | |
| 11750A-F2 | VGB6 | C57BL/6NTac | |
