| Gene | Dnase1 |
| Chromosome | 16 |
| Deletion Start | 4,037,554 |
| Deletion End | 4,039,865 |
| Deletion Size | 2,312 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 4,038,270 | 4,038,292 | GGTAAAGCCCTATGCTGCCAAAG |
| TUP | 4,038,295 | 4,038,322 | TGAGAATAAACTCAGGGATGTGCAGGCC |
| TUR | 4,038,352 | 4,038,369 | TCACCTGGCCCTCCAAAC |
| TDF | 4,039,079 | 4,039,100 | GGTTCTATTGGCCCTGTTTGTC |
| TDP | 4,039,101 | 4,039,123 | CCTGGACCAACAGGCATGTCTGC |
| TDR | 4,039,148 | 4,039,165 | GCAGCCAGCATTGAAGTC |
PCR Primers
| Primer | Sequence |
| SU | CTCATCATCTCTGTAGACATC |
| SD | AACACTGACAGCCAAGACAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 100 bp | + | + | - |
| TDF | TDR | 87 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11756A-B3 | VGB6 | C57BL/6NTac | |
| 11756A-D4 | VGB6 | C57BL/6NTac | |
| 11756A-F9 | VGB6 | C57BL/6NTac | |
| 11756A-C7 | VGB6 | C57BL/6NTac | |
