| Gene | 1700027D21Rik |
| Chromosome | 10 |
| Deletion Start | 76,562,369 |
| Deletion End | 76,570,008 |
| Deletion Size | 7,640 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 76,562,638 | 76,562,659 | GGCTATGCTGGTTGGTAATGTC |
| TUP | 76,562,660 | 76,562,683 | TCAAAGCACAGCCACCTGGCTTAA |
| TUR | 76,562,696 | 76,562,716 | AGCCTGGAGCCCTGGAATCAC |
| TDF | 76,569,620 | 76,569,641 | GCTTTCCTGACCTGGTTTCTTG |
| TDP | 76,569,642 | 76,569,665 | TTTCCTCTGCTCCGTGTCCCACAG |
| TDR | 76,569,695 | 76,569,715 | TCACGCAGCTTCTTCTCATCC |
PCR Primers
| Primer | Sequence |
| SU | AGCCGGTGTTATGAACACTC |
| SD | GGTTCTGCTCAGAAACACCA |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 96 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11759A-A2 | VGB6 | C57BL/6NTac | |
| 11759A-A6 | VGB6 | C57BL/6NTac | |
| 11759A-C5 | VGB6 | C57BL/6NTac | |
| 11759A-D7 | VGB6 | C57BL/6NTac | |
| 11759A-F11 | VGB6 | C57BL/6NTac | |
| 11759A-G10 | VGB6 | C57BL/6NTac | |
