| Gene | Star |
| Chromosome | 8 |
| Deletion Start | 25,808,572 |
| Deletion End | 25,812,888 |
| Deletion Size | 4,317 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 25,809,317 | 25,809,338 | AGAGATGTGGCTGGAGTTTGAG |
| TUP | 25,809,339 | 25,809,360 | TCCCTGCTGGGATCTGTGTCCC |
| TUR | 25,809,394 | 25,809,413 | CTCTGGGAGAGGGACAGTTC |
| TDF | 25,812,747 | 25,812,767 | TCCCAGTGCTCTTGTGTCTAC |
| TDP | 25,812,776 | 25,812,802 | TGGCTGCCGAAGACAATCATCAACCAG |
| TDR | 25,812,820 | 25,812,839 | GGTGGTTGGCGAACTCTATC |
PCR Primers
| Primer | Sequence |
| SU | TACCTGCAGAGTCTGGTCC |
| SD | GTCAGCATATACAAGCCTAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 97 bp | + | + | - |
| TDF | TDR | 93 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11760A-H10 | VGB6 | C57BL/6NTac | |
| 11760C-G4 | VGB6 | C57BL/6NTac | |
| 11760C-F11 | VGB6 | C57BL/6NTac | |
| 11760C-B12 | VGB6 | C57BL/6NTac | |
