11761   Olig1     Status: ES cell colonies screened / QC positives

GeneOlig1
Chromosome16
Deletion Start91,269,880
Deletion End91,270,715
Deletion Size836
Genome Build38
MGI IDMGI:1355334
NCBI Gene ID50914
BAC ID382G11
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 16

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF91,270,15991,270,178CTGCGGCGCAAGATCAACAG
TUP91,270,18691,270,208CGGAAGCGCATGCAGGACCTGAA
TUR91,270,23191,270,253GCCGAGTAGGGTAGGATAACTTC
TDF91,270,48991,270,509CCCACCAAGTACCTGTCTCTA
TDP91,270,51391,270,532CTCGACGAGCCACCGTGCGG
TDR91,270,57891,270,598AACTTGCACACCGCACAGGAG

PCR Primers

PrimerSequence
SUACTCTGGCTGTACCTCTGC
SDGCTCACGGATACGAGAATAG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR95 bp++-
TDFTDR110 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
11761A-B10VGB6C57BL/6NTac
11761A-C4VGB6C57BL/6NTac
11761A-D8VGB6C57BL/6NTac
11761A-C7VGB6C57BL/6NTac
11761A-A11VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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