| Gene | Myo1a |
| Chromosome | 10 |
| Deletion Start | 127,705,259 |
| Deletion End | 127,720,747 |
| Deletion Size | 15,489 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 127,705,833 | 127,705,853 | GGCAAATGTGGCCTACCAGTC |
| TUP | 127,705,862 | 127,705,885 | ATCGGGACCGGGACCAGTGTATCC |
| TUR | 127,705,914 | 127,705,931 | TGAGCCTCCTCACCAGTC |
| TDF | 127,719,909 | 127,719,929 | TCTCACGGATGCGAAGAAGTC |
| TDP | 127,719,931 | 127,719,956 | CAGGCCCAGATTGTCATAGGACTGGA |
| TDR | 127,719,988 | 127,720,008 | CAAGCTAAAGAGCCCGTCCTG |
PCR Primers
| Primer | Sequence |
| SU | TTCCAGCTTTCTCCTTTAAGC |
| SD | GCATATGTATCTGAGCAAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 99 bp | + | + | - |
| TDF | TDR | 100 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11762A-B1 | VGB6 | C57BL/6NTac | |
| 11762A-F8 | VGB6 | C57BL/6NTac | |
