| Gene | Psph |
| Chromosome | 5 |
| Deletion Start | 129,786,991 |
| Deletion End | 129,781,156 |
| Deletion Size | 5,836 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 129,770,940 | 129,770,961 | CAAGGTGCTTGTGTACGGGATG |
| TUP | 129,770,916 | 129,770,939 | TGTTGGTCCGCAGTACATGACGCC |
| TUR | 129,770,882 | 129,770,901 | CAGGAGGTCGGCTTGAGAAC |
| TDF | 129,765,879 | 129,765,900 | AGGATGCTTTCATTGGCTTTGG |
| TDP | 129,765,847 | 129,765,875 | CAATGTGATCAGGCAGCAGGTTAAGGACA |
| TDR | 129,765,808 | 129,765,826 | CCAGCAGCTCCACGAAGTC |
PCR Primers
| Primer | Sequence |
| SU | CACTGCCCAGCAACTCTATT |
| SD | TACTGAATCCATGTTGCAAGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 80 bp | + | + | - |
| TDF | TDR | 93 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11763B-C12 | VGB6 | C57BL/6NTac | |
| 11763B-G12 | VGB6 | C57BL/6NTac | |
| 11763B-G7 | VGB6 | C57BL/6NTac | |
| 11763B-G2 | VGB6 | C57BL/6NTac | |
| 11763C-D10 | VGB6 | C57BL/6NTac | |
| 11763C-D1 | VGB6 | C57BL/6NTac | |
