| Gene | Tymp |
| Chromosome | 15 |
| Deletion Start | 89,375,538 |
| Deletion End | 89,372,225 |
| Deletion Size | 3,314 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 89,376,279 | 89,376,302 | AGGAGGTGTGGGTGACAAGGTTAG |
| TUP | 89,376,257 | 89,376,278 | CCTGGTTCTGGCACCTGCCCTG |
| TUR | 89,376,202 | 89,376,221 | AGCGGGTCAGAGGTCTGTAG |
| TDF | 89,373,690 | 89,373,707 | GACGTGGGCCAGTGTTTG |
| TDP | 89,373,666 | 89,373,688 | CCCGAGGTAAGCGCTGTCTGGAA |
| TDR | 89,373,609 | 89,373,627 | GAGCGACAGAGGCGGTTAC |
PCR Primers
| Primer | Sequence |
| SU | TTGAATAGGTACTAGACGTGG |
| SD | ACAACCTGGATCTCTTATGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 101 bp | + | + | - |
| TDF | TDR | 99 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11770A-G12 | VGB6 | C57BL/6NTac | |
| 11770A-H9 | VGB6 | C57BL/6NTac | |
| 11770A-F4 | VGB6 | C57BL/6NTac | |
