11771 Arf2 Status: ES cell colonies screened / QC no positives
| Gene | Arf2 |
| Chromosome | 11 |
| Deletion Start | 103,969,101 |
| Deletion End | 103,983,769 |
| Deletion Size | 14,669 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 103,970,003 | 103,970,024 | CACTGCCTTTCTCAGTCTCCAC |
| TUP | 103,970,025 | 103,970,045 | CCTCCCTGGCTTCCCTTGGGA |
| TUR | 103,970,047 | 103,970,067 | CACACCTGGCTCCGAGAGATG |
| TDF | 103,983,642 | 103,983,661 | TCCCTTCGCCAGAGAAACTG |
| TDP | 103,983,664 | 103,983,688 | ACATTCAGGCTACCTGTGCGACCAG |
| TDR | 103,983,693 | 103,983,711 | GGCCTTCGTAAAGCCCATC |
PCR Primers
| Primer | Sequence |
| SU | TTCTGGCAGAGTATCAGAGG |
| SD | CTAGAGATTCTCTGGCTATC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 65 bp | + | + | - |
| TDF | TDR | 70 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
