| Gene | Slbp |
| Chromosome | 5 |
| Deletion Start | 33,646,644 |
| Deletion End | 33,635,205 |
| Deletion Size | 11,440 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 33,652,213 | 33,652,232 | CCACCCGGTTATGGGAGTCG |
| TUP | 33,652,169 | 33,652,193 | TAAGCGGGTCATGCGGTCTACGAGT |
| TUR | 33,652,143 | 33,652,162 | AGCTTACAGACGGCGAGAAG |
| TDF | 33,641,547 | 33,641,565 | TGTGCAGTCCTGGTTGTTC |
| TDP | 33,641,519 | 33,641,546 | TGGAACTCACTGTAGAAACCAGTTTGGC |
| TDR | 33,641,464 | 33,641,484 | CAGGCAAATCCCAGCATGAAG |
PCR Primers
| Primer | Sequence |
| SU | GCGTTGTAGTTTAGCGACTG |
| SD | CTGAAGGCTCAAGTTTCCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 90 bp | + | + | - |
| TDF | TDR | 102 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11777A-B11 | VGB6 | C57BL/6NTac | |
| 11777A-C8 | VGB6 | C57BL/6NTac | |
| 11777A-D4 | VGB6 | C57BL/6NTac | |
| 11777A-F8 | VGB6 | C57BL/6NTac | |
| 11777A-D7 | VGB6 | C57BL/6NTac | |
