| Gene | Mrpl37 |
| Chromosome | 4 |
| Deletion Start | 107,066,792 |
| Deletion End | 107,056,022 |
| Deletion Size | 10,771 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 107,066,472 | 107,066,494 | AAGAACCCTGCTACATCTTCCAC |
| TUP | 107,066,452 | 107,066,471 | CAGCGTTGCCGACTCCTGGA |
| TUR | 107,066,422 | 107,066,440 | TCTCCTCCCGTTTGTCCTG |
| TDF | 107,056,197 | 107,056,217 | GATGCAGGAGATGACAGTGAC |
| TDP | 107,056,176 | 107,056,196 | TCGCCAGGCACTGGTGACTCT |
| TDR | 107,056,144 | 107,056,164 | TCATGGCAGCAAAGGAAATGG |
PCR Primers
| Primer | Sequence |
| SU | AATGCCTAAGGGTCAAAGGC |
| SD | TGAAGAAACTCAGGCATCGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 73 bp | + | + | - |
| TDF | TDR | 74 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11781A-C8 | VGB6 | C57BL/6NTac | |
| 11781A-C12 | VGB6 | C57BL/6NTac | |
| 11781A-E5 | VGB6 | C57BL/6NTac | |
| 11781A-G3 | VGB6 | C57BL/6NTac | |
| 11781A-F8 | VGB6 | C57BL/6NTac | |
| 11781A-H12 | VGB6 | C57BL/6NTac | |
