| Gene | Gadd45a |
| Chromosome | 6 |
| Deletion Start | 67,037,241 |
| Deletion End | 67,035,658 |
| Deletion Size | 1,584 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 67,036,579 | 67,036,596 | TAGGGTACGGAGCGTGTC |
| TUP | 67,036,558 | 67,036,578 | TAAGCTCGTGGGTGGCCTCCA |
| TUR | 67,036,494 | 67,036,515 | GTGCAGAGGTAAGCAGAGGTAG |
| TDF | 67,036,385 | 67,036,408 | TCATTCTTCATTTGCTGTCAAAGC |
| TDP | 67,036,355 | 67,036,383 | AAAGGTAGAAGTGGTGTAGGAGACAGACC |
| TDR | 67,036,306 | 67,036,326 | TCCACTCGGTACACTTCAGAG |
PCR Primers
| Primer | Sequence |
| SU | AACAATTGGCAGGCTGTTTC |
| SD | TGAAAGTAACCTGGCCATCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 103 bp | + | + | - |
| TDF | TDR | 103 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11783A-B5 | VGB6 | C57BL/6NTac | |
| 11783A-C9 | VGB6 | C57BL/6NTac | |
| 11783A-F7 | VGB6 | C57BL/6NTac | |
| 11783A-D12 | VGB6 | C57BL/6NTac | |
