| Gene | Tmem168 |
| Chromosome | 6 |
| Deletion Start | 13,603,362 |
| Deletion End | 13,582,787 |
| Deletion Size | 20,576 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 13,602,906 | 13,602,924 | CGCCATCGGCCTACTTTAC |
| TUP | 13,602,875 | 13,602,904 | AACCACAGTGGAGTTTCTGGAACTTGTTGG |
| TUR | 13,602,831 | 13,602,854 | TCAGAGACTTCTCCACCAACATAG |
| TDF | 13,583,155 | 13,583,174 | TGAGCTGGCGAAGACAGTAG |
| TDP | 13,583,128 | 13,583,154 | ATATTGAAGAAGCTGACCCGCCACAGC |
| TDR | 13,583,067 | 13,583,091 | CAGCAGATGTTGTTTGTGGAGTTAC |
PCR Primers
| Primer | Sequence |
| SU | TCAGTGTGTCAAAGGCAAGTG |
| SD | GCCACTCCAGAGCACAGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 94 bp | + | + | - |
| TDF | TDR | 108 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11785A-B9 | VGB6 | C57BL/6NTac | |
| 11785A-H1 | VGB6 | C57BL/6NTac | |
| 11785A-F6 | VGB6 | C57BL/6NTac | |
| 11785A-G12 | VGB6 | C57BL/6NTac | |
