| Gene | 3200002M19Rik |
| Chromosome | 7 |
| Deletion Start | 101,897,798 |
| Deletion End | 101,899,271 |
| Deletion Size | 1,474 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 101,898,085 | 101,898,102 | CAGTGCAGCATGGAGGTC |
| TUP | 101,898,103 | 101,898,123 | TACCACCGGGCCGTGAGCTTG |
| TUR | 101,898,125 | 101,898,144 | AAGCAGGTCTCCAGCGTAAG |
| TDF | 101,898,561 | 101,898,583 | GCATCGCAGAGAAAGACAACAAC |
| TDP | 101,898,591 | 101,898,612 | CAATTGGCAAGCCGGCCTCAGA |
| TDR | 101,898,614 | 101,898,633 | CACCCACAGGAACCACTCAC |
PCR Primers
| Primer | Sequence |
| SU | CTGCTATTGCTAATCTTGTGC |
| SD | ACCAATCACAGAAACCTGGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 60 bp | + | + | - |
| TDF | TDR | 73 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11786A-E1 | VGB6 | C57BL/6NTac | |
| 11786A-E9 | VGB6 | C57BL/6NTac | |
| 11786A-F7 | VGB6 | C57BL/6NTac | |
| 11786A-H8 | VGB6 | C57BL/6NTac | |
| 11786A-G2 | VGB6 | C57BL/6NTac | |
| 11786A-F12 | VGB6 | C57BL/6NTac | |
