| Gene | Casc3 |
| Chromosome | 11 |
| Deletion Start | 98,809,867 |
| Deletion End | 98,831,646 |
| Deletion Size | 21,780 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 98,810,156 | 98,810,174 | ATGCCGGGCCTCCTCTTAG |
| TUP | 98,810,179 | 98,810,204 | AAAGAAAGGTTGTCCCGCTGCGAATC |
| TUR | 98,810,231 | 98,810,250 | TCAGAAACCACCCAGTCCTC |
| TDF | 98,831,409 | 98,831,427 | AGGTACGGCTCCTCCCACT |
| TDP | | | TCCTAGCATGTGGGTG |
| TDR | 98,831,449 | 98,831,470 | GAAGACAGACCCTCTGGAGAAA |
PCR Primers
| Primer | Sequence |
| SU | AGAAGAGCTCGAAACCCTCC |
| SD | AGAGCCACAGAGCCACTTTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 95 bp | + | + | - |
| TDF | TDR | 62 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11788B-H10 | VGB6 | C57BL/6NTac | |
| 11788A-E8 | VGB6 | C57BL/6NTac | |
