| Gene | Dpf1 |
| Chromosome | 7 |
| Deletion Start | 29,304,084 |
| Deletion End | 29,316,581 |
| Deletion Size | 12,498 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 29,304,647 | 29,304,668 | CAGACCTAGAAGTCCCGATGTC |
| TUP | 29,304,669 | 29,304,690 | TCGGTCGGCTTGGACCACTGTA |
| TUR | 29,304,715 | 29,304,734 | GGAGGTGCTCGCGTTAGATG |
| TDF | 29,315,989 | 29,316,009 | TGAGGTCACCAAGGTCAGAAG |
| TDP | 29,316,012 | 29,316,036 | TGTAGGGCCAGGATTTGAACCTCCG |
| TDR | 29,316,037 | 29,316,055 | GCGGGCCAGATACCAACTC |
PCR Primers
| Primer | Sequence |
| SU | TGGGAAGGATGCGTTAGTAG |
| SD | GCTGGCTAGCATCCACTAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 88 bp | + | + | - |
| TDF | TDR | 67 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11792A-E10 | VGB6 | C57BL/6NTac | |
| 11792A-A10 | VGB6 | C57BL/6NTac | |
