| Gene | Trap1a |
| Chromosome | X |
| Deletion Start | 139,333,957 |
| Deletion End | 139,337,048 |
| Deletion Size | 3,092 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 139,334,368 | 139,334,385 | GCCGGAGCTGAGGAAATG |
| TUP | 139,334,388 | 139,334,408 | TGCTGGCGCTAACTGTGCCTG |
| TUR | 139,334,449 | 139,334,473 | CAGATGTGGGCAAGAGTAAAGAATG |
| TDF | 139,336,374 | 139,336,393 | TCAGCAGGAAAGGCTGGATG |
| TDP | 139,336,397 | 139,336,425 | ATAAGGCCAGGTTCTGAGGACAGCTGGAA |
| TDR | 139,336,432 | 139,336,455 | ACTTAGGGTTATAGGAGCCACTTG |
PCR Primers
| Primer | Sequence |
| SU | CAATGAGCTTACTGTTCTCG |
| SD | TCTCTACTCCCGAGTCTAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 106 bp | + | + | - |
| TDF | TDR | 82 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11794A-C10 | VGB6 | C57BL/6NTac | |
| 11794B-B1 | VGB6 | C57BL/6NTac | |
| 11794B-E10 | VGB6 | C57BL/6NTac | |
| 11794B-G9 | VGB6 | C57BL/6NTac | |
| 11794C-G3 | VGB6 | C57BL/6NTac | |
| 11794C-G5 | VGB6 | C57BL/6NTac | |
