| Gene | Gadd45g |
| Chromosome | 13 |
| Deletion Start | 51,846,783 |
| Deletion End | 51,847,992 |
| Deletion Size | 1,210 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 13
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 51,846,919 | 51,846,937 | TGCACTGTCTGGCTGTGAG |
| TUP | 51,846,941 | 51,846,962 | CCGAGGCGCGGGACCAGAATTA |
| TUR | 51,847,002 | 51,847,022 | CCCAACTACGATCAGGTGCTG |
| TDF | 51,847,525 | 51,847,547 | TCTGACCTTGTCCTTCCCATCTG |
| TDP | 51,847,549 | 51,847,572 | TCTGCAGGGACCCTGACAATGTGA |
| TDR | 51,847,599 | 51,847,619 | CGCTATGTCGCCCTCATCTTC |
PCR Primers
| Primer | Sequence |
| SU | GGCAGATTTGAGGCTGTGTC |
| SD | AACGTCCTCGCCTAGGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 104 bp | + | + | - |
| TDF | TDR | 95 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11796A-E4 | VGB6 | C57BL/6NTac | |
| 11796A-F5 | VGB6 | C57BL/6NTac | |
| 11796A-F7 | VGB6 | C57BL/6NTac | |
| 11796A-G9 | VGB6 | C57BL/6NTac | |
| 11796A-F11 | VGB6 | C57BL/6NTac | |
