| Gene | Dnajb6 |
| Chromosome | 5 |
| Deletion Start | 29,748,374 |
| Deletion End | 29,785,216 |
| Deletion Size | 36,843 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 29,748,734 | 29,748,757 | CATGATAGGCAGACACCAGTTTAC |
| TUP | 29,748,758 | 29,748,781 | TGCCCAACACAGACCTAAAGTGGC |
| TUR | 29,748,843 | 29,748,867 | CACCAACTAGATAGAATGCCCAATG |
| TDF | 29,784,991 | 29,785,011 | GTTGCTGGTGTGCAGTGTTTC |
| TDP | 29,785,020 | 29,785,047 | TGTTTCCTTAGGGCTCAAAGAAGGTGGC |
| TDR | 29,785,050 | 29,785,069 | TCTGCTTCTGCTTCTTCCTC |
PCR Primers
| Primer | Sequence |
| SU | GCACCTGATCTCTGCCTCAG |
| SD | AAACTGCGCACTGTACCACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 134 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11798A-A5 | VGB6 | C57BL/6NTac | |
| 11798A-D6 | VGB6 | C57BL/6NTac | |
| 11798A-E1 | VGB6 | C57BL/6NTac | |
| 11798A-G7 | VGB6 | C57BL/6NTac | |
