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Sequence Downloads and Strategy Information
Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 115,518,415 | 115,518,438 | GTGAAAGCTGTCCAGTTCTACCTC |
| TUP | 115,518,439 | 115,518,466 | CACAGGCAATGGCTACTCAAGGCTTTCC |
| TUR | 115,518,524 | 115,518,546 | CCTTCTCTTCCACCCATCCTTCT |
| TDF | CATGCCCTTAGCCCGAC | ||
| TDP | 115,532,648 | 115,532,676 | TGTGCTGAAGTCCAAAAATGGGATCTACC |
| TDR | 115,532,687 | 115,532,709 | CTTACCAGAATCAGTGGAGCTTC |
PCR Primers
| Primer | Sequence |
| SU | ATGCATGCAGTACAGGTGTG |
| SD | ACAAATGCTGTGCATGACAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 132 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the KOMP Repository.| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11799A-D6 | VGB6 | C57BL/6NTac | |
| 11799A-E2 | VGB6 | C57BL/6NTac | |
| 11799A-H6 | VGB6 | C57BL/6NTac |
