| Gene | Cdc42ep4 |
| Chromosome | 11 |
| Deletion Start | 113,750,221 |
| Deletion End | 113,749,171 |
| Deletion Size | 1,051 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 113,729,151 | 113,729,170 | AAGCTGCCCAAGAGCCTGTC |
| TUP | 113,729,127 | 113,729,149 | TCCAGCCCTGTGAAGAAGGCAGA |
| TUR | 113,729,104 | 113,729,121 | TCTTCGGGCCACCATCTC |
| TDF | 113,729,005 | 113,729,023 | CTGCCCATCATGCCCAAAG |
| TDP | 113,728,982 | 113,729,003 | CAGCTACGGGCTGAAGCATGCA |
| TDR | 113,728,951 | 113,728,970 | CCAGGTCGATGTGGAAGGAC |
PCR Primers
| Primer | Sequence |
| SU | GTTAGGAATTCACTGCTGGTG |
| SD | ACATCTGGCATCCATCTGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 67 bp | + | + | - |
| TDF | TDR | 73 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11802A-H10 | VGB6 | C57BL/6NTac | |
| 11802C-A8 | VGB6 | C57BL/6NTac | |
| 11802C-C5 | VGB6 | C57BL/6NTac | |
| 11802C-E12 | VGB6 | C57BL/6NTac | |
