| Gene | Socs3 |
| Chromosome | 11 |
| Deletion Start | 117,967,773 |
| Deletion End | 117,967,048 |
| Deletion Size | 726 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 117,967,989 | 117,968,006 | CCAGCGCCACTTCTTCAC |
| TUP | 117,967,965 | 117,967,987 | TTGAGCGTCAAGACCCAGTCGGG |
| TUR | 117,967,945 | 117,967,964 | GGATGCGTAGGTTCTTGGTC |
| TDF | 117,967,651 | 117,967,671 | CCACCCTCCAGCATCTTTGTC |
| TDP | 117,967,625 | 117,967,648 | AAGACTGTCAACGGCCACCTGGAC |
| TDR | 117,967,561 | 117,967,585 | GAGCATCATACTGATCCAGGAACTC |
PCR Primers
| Primer | Sequence |
| SU | ACCGAGGACAGTCGAGTTCC |
| SD | AGAGTCCGCTTGTCAAAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 62 bp | + | + | - |
| TDF | TDR | 111 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11805A-A9 | VGB6 | C57BL/6NTac | |
| 11805D-D11 | VGB6 | C57BL/6NTac | |
| 11805E-A3 | VGB6 | C57BL/6NTac | |
| 11805F-A3 | VGB6 | C57BL/6NTac | |
| 11805F-A5 | VGB6 | C57BL/6NTac | |
| 11805F-C5 | VGB6 | C57BL/6NTac | |
| 11805F-C9 | VGB6 | C57BL/6NTac | |
