| Gene | Lcat |
| Chromosome | 8 |
| Deletion Start | 105,943,405 |
| Deletion End | 105,939,581 |
| Deletion Size | 3,825 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 105,942,869 | 105,942,890 | TGGCCTCTGTCTTACCAAGAAC |
| TUP | 105,942,838 | 105,942,863 | ACCCAGCCAGAGATACCCAGCTATCC |
| TUR | 105,942,812 | 105,942,833 | ACAGACCTCTCCTGCTATCCTG |
| TDF | 105,940,152 | 105,940,173 | CTCCCTGTCCCACCTTGTTCTC |
| TDP | 105,940,123 | 105,940,146 | TCCTAGGTGACAACCAGGGCATCC |
| TDR | 105,940,079 | 105,940,100 | GGTTATGCGCTGCTCCTCTTTC |
PCR Primers
| Primer | Sequence |
| SU | CCTGCTGCTCCGGCTCTTT |
| SD | CTGAGCAGCTCAACTAGGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 95 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11806A-A2 | VGB6 | C57BL/6NTac | |
| 11806A-D3 | VGB6 | C57BL/6NTac | |
| 11806A-H11 | VGB6 | C57BL/6NTac | |
| 11806A-A10 | VGB6 | C57BL/6NTac | |
