| Gene | Apitd1 |
| Chromosome | 4 |
| Deletion Start | 149,135,927 |
| Deletion End | 149,127,178 |
| Deletion Size | 8,750 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 149,137,218 | 149,137,240 | GAGGGAATCTATGGGTTCGAGTC |
| TUP | 149,137,194 | 149,137,215 | AGCACCGCACCTACCGGGCTTG |
| TUR | 149,137,174 | 149,137,193 | GGGTCACAGTCGTTCACCAC |
| TDF | 149,129,225 | 149,129,246 | GGTGAAGACACTTGCCACTTTG |
| TDP | 149,129,200 | 149,129,224 | AGGTGTCAGTGTCACCTGGCTGTGA |
| TDR | 149,129,147 | 149,129,167 | CATGAGAAGGGCAGAGACCAG |
PCR Primers
| Primer | Sequence |
| SU | CACGTTCTTACAGATGCTTG |
| SD | AGTCACTAAGTCACGCTGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 67 bp | + | + | - |
| TDF | TDR | 100 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11809B-G7 | VGB6 | C57BL/6NTac | |
| 11809B-B11 | VGB6 | C57BL/6NTac | |
| 11809B-B6 | VGB6 | C57BL/6NTac | |
| 11809B-G6 | VGB6 | C57BL/6NTac | |
| 11809A-D8 | VGB6 | C57BL/6NTac | |
